In 1934 at the University of Basel under Eugen Ludwig, Josef Klingler developed a new method of dissection based on a freezing technique for brain tissue that eloquently revealed the white matter tracts.
Torkildsen A. The Gross Anatomy of the Lateral Ventricles. J Anat. 1934 Jul;68(Pt 4):480-91. PubMed PMID: 17104497; PubMed Central PMCID: PMC1249003 1).