see also Multiparameter flow cytometry.
All brain biopsies performed between 2010 and 2015 at Brain Tumor Center, University Medical Center Rotterdam and analyzed by both immunohistochemistry and flow cytometry were included in a retrospective study. Immunohistochemistry was considered the gold standard.
In a total of 77 biopsies from 71 patients, 49 lymphomas were diagnosed by immunohistochemistry, flow cytometry results were concordant in 71 biopsies (92,2%). van der Meulen et al., found a specificity and sensitivity of flow cytometry of 100% and 87,8%, respectively. The time between the biopsy and reporting the result (turnaround time) was significantly shorter for flow cytometry, compared to immunohistochemistry (median: 1 versus 5 days).
Flow cytometry has a high specificity and can confirm the diagnosis of a lymphoma significantly faster than immunohistochemistry. This allows for rapid initiation of treatment in this highly aggressive tumor. However, since its sensitivity is less than 100%, van der Meulen et al., recommend to perform histology plus immunohistochemistry in parallel to flow cytometry 1).