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u87

U87

In cell biology, U87 is a human primary glioblastoma cell line formally known as U-87 MG. It has epithelial morphology, and was obtained from a stage four 44 year-old cancer patient.

U-87 MG can be obtained from the American Type Culture Collection (ATCC) where it is known by the accession number HTB-14.

The entire sequence of the genome of U-87 MG has been published in PLoS Genetics, 2010 January; 6(1): e1000832.

Growth conditions U87 growth media is generally made with Eagle's minimum essential medium + 10% FBS + 100 U/ml penicillin + 100 ug/ml streptomycin.

It is propagated at 37 degrees Celsius in a 5% carbon dioxide atmosphere.

DNA profile of the widely used glioma cell line U87MG is different from that of the original cells and that it is likely to be a bona fide human glioblastoma cell line of unknown origin 1).

1)
Allen M, Bjerke M, Edlund H, Nelander S, Westermark B. Origin of the U87MG glioma cell line: Good news and bad news. Sci Transl Med. 2016 Aug 31;8(354):354re3. doi: 10.1126/scitranslmed.aaf6853. PubMed PMID: 27582061.
u87.txt · Last modified: 2017/07/22 15:59 by administrador