In cell biology, U87 is a human primary glioblastoma cell line formally known as U-87 MG. It has epithelial morphology, and was obtained from a stage four 44 year-old cancer patient.
U-87 MG can be obtained from the American Type Culture Collection (ATCC) where it is known by the accession number HTB-14.
The entire sequence of the genome of U-87 MG has been published in PLoS Genetics, 2010 January; 6(1): e1000832.
Growth conditions U87 growth media is generally made with Eagle's minimum essential medium + 10% FBS + 100 U/ml penicillin + 100 ug/ml streptomycin.
It is propagated at 37 degrees Celsius in a 5% carbon dioxide atmosphere.
DNA profile of the widely used glioma cell line U87MG is different from that of the original cells and that it is likely to be a bona fide human glioblastoma cell line of unknown origin 1).