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astrocyte_elevated_gene_1

mir 96 has been reported to exhibit a correlation with the Epithelial mesenchymal transition (EMT) process. However, the functional role of miR‑96 and its mechanism in glioblastoma multiforme (GBM) remains to be completely elucidated. The objective of a study was to investigate the functional role and mechanism of miR‑96 in the migration and invasion, in addition to proliferation, apoptosis and cell cycle distribution, of GBM. In a study, the results suggested that the introduction of miR‑96 significantly inhibited the migration and invasion, in addition to proliferation and cell cycle progression, of GBM cells and promoted their apoptosis in vitro, leading to the hypothesis that miR‑96 may be a potential tumor suppressor. It was subsequently confirmed that astrocyte elevated gene 1 (AEG‑1) was a direct target gene of miR‑96, using a luciferase assay and reverse transcription‑quantitative polymerase chain reaction analysis, in addition to western blotting. miR‑96 was observed to downregulate the expression of AEG‑1 at the mRNA and protein levels. Notably, AEG‑1 may suppress EMT by increasing the expression levels of E‑cadherin, an epithelial marker, and decreasing the expression levels of vimentin, a mesenchymal marker. Therefore, it was concluded that miR‑96 may impede the EMT process by downregulating AEG‑1 in GBM. Additionally, it was observed that inhibition of AEG‑1 led to a similar effect compared with overexpression of miR‑96 in GBM. In conclusion, the results of the present study demonstrated that miR‑96 may act as a tumor suppressor by regulating EMT via targeting of AEG‑1, suggesting that miR‑96 may be a potential biomarker and anticancer therapeutic target for GBM in the future 1).

1)
Feng S, Yao J, Zhang Z, Zhang Y, Zhang Z, Liu J, Tan W, Sun C, Chen L, Yu X. miR‑96 inhibits EMT by targeting AEG‑1 in glioblastoma cancer cells. Mol Med Rep. 2017 Dec 8. doi: 10.3892/mmr.2017.8227. [Epub ahead of print] PubMed PMID: 29257267.
astrocyte_elevated_gene_1.txt · Last modified: 2017/12/21 08:35 by administrador