Liquid biopsy

Liquid biopsy is a developing field of diagnosis and patient follow-up in multiple types of cancer. Fragments of circulating nucleic acids are collected in various forms from different bodily fluids, including serum, urine, or cerebrospinal fluid in order to measure the quality and quantity of these markers. Multiple types of nucleic acids can be analyzed using liquid biopsy. Circulating cell-free DNA, mitochondrial DNA, or the more stable long and small non-coding RNAs, circular RNAs, or microRNAs can be identified and measured by novel PCR and next-generation sequencing-based methods. These markers can be used to detect the previously described alterations in a minimally invasive method. These markers can be used to differentiate patients with poor or better prognosis or to identify patients who do not respond to therapy.

Liquid biopsies collect and analyze tumor components in body fluids, and there is an increasing interest in the investigation of liquid biopsies as a surrogate for tumor tissue in the management of both primary and secondary brain tumors 1).

Boire et al., critically reviewed available literature on spinal fluid and plasma circulating tumor cells (CTCs) and cell-free tumor (ctDNA) for diagnosis and monitoring of leptomeningeal and parenchymal brain metastases.

They discuss technical issues and propose several potential applications of liquid biopsies in different clinical settings, i.e. for initial diagnosis, for assessment during treatment and for guidance of treatment decisions. Last, ongoing clinical studies on CNS metastases, that include liquid biopsies, are summarized and recommendations for future clinical studies are provided 2).

see Liquid biopsy for intracranial metastases diagnosis

Shankar GM, Balaj L, Stott SL, Nahed B, Carter BS. Liquid biopsy for brain tumors. Expert Rev Mol Diagn. 2017 Oct;17(10):943-947. doi: 10.1080/14737159.2017.1374854. Epub 2017 Sep 6. Review. PubMed PMID: 28875730; PubMed Central PMCID: PMC5856481.
Boire A, Brandsma D, Brastianos PK, Le Rhun E, Ahluwalia M, Junk L, Glantz M, Groves MD, Lee EQ, Lin N, Raizer J, Rudà R, Weller M, van den Bent MJ, Vogelbaum MA, Chang S, Wen PY, Soffietti R. Liquid biopsy in Central Nervous System metastases: a RANO review and proposals for clinical applications. Neuro Oncol. 2019 Jan 22. doi: 10.1093/neuonc/noz012. [Epub ahead of print] PubMed PMID: 30668804.
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  • Last modified: 2021/02/22 21:23
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