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Department of Neurosurgery, Renmin Hospital, Hubei University of Medicine, Shiyan, China.

Contact urticaria is recognized as the wheal and flare reaction at a site from direct contact with a chemical or protein agent. Ongoing studies have proposed that gene silencing may have a promising future in finding optimal treatment of a variety of disease; hence, the aim of the study was to investigate the effect of RNA interference-mediated E-selectin ( SELE) gene silencing on cell adhesion molecule expression and on cell-cell adhesion in vascular endothelial cells (VECs) in a mouse model of immunologic contact urticaria (ICU). Following the successful establishment of mouse models of ICU induced by antidinitrophenol immunoglobulin E (IgE) combining 2,4-dinitrofluorobenzene challenge, enzyme-linked immunosorbent assay and immunohistochemistry were used to measure the levels of IgE, interleukin 4 (IL-4), interferon-γ (IFN-γ), and histamine as well as the positive expression rate of SELE, respectively. The siRNA- SELE vector was constructed and transfection efficiency was estimated prior to performing quantitative reverse-transcription polymerase chain reaction and Western blot assay to determine the relative expression of SELE, eosinophil cationic protein (ECP), intercellular adhesion molecule 1 (ICAM-1), L-selectin (CD62L), and the alpha chain of leukocyte function-associated antigen-1 (CD11a). Adhesion assay was then performed to assess the cell adhesion ability in VECs. Elevated levels of IgE, IL-4, IFN-γ, and histamine and increased positive expression rate of SELE were indicative of successful establishment of mouse models of ICU. Furthermore, the relative expression levels of SELE, ECP, ICAM-1, CD62L, and CD11a were highest in the OE- SELE group. Besides, cell adhesion ability of VECs was notably promoted. Collectively, the current study define the potential role of SELE silencing as an inhibitor to ICU development by inhibiting cell adhesion ability of VECs 1).

2: Fu W, Cao S, Liu B, Li H, Song F, Gan Y, Li W, Wang L, Opoku S, Yan S, Yue W, Yan F, Wang C, Li H, Liu Q, Wang X, Wang Z, Lu Z. Association of general and central adiposity with blood pressure among Chinese adults: results from the China National Stroke Prevention Project. J Hypertens. 2018 Jul 16. doi: 10.1097/HJH.0000000000001852. [Epub ahead of print] PubMed PMID: 30020239.

3: Chen W, Sinha B, Li Y, Benowitz L, Chen Q, Zhang Z, Patel NJ, Aziz-Sultan AM, Chiocca AE, Wang X. Monogenic, Polygenic, and MicroRNA Markers for Ischemic Stroke. Mol Neurobiol. 2018 Jun 8. doi: 10.1007/s12035-018-1055-3. [Epub ahead of print] Review. PubMed PMID: 29948938.

4: Li G, Huang M, Cai Y, Ke Y, Yang Y, Sun X. miR‑141 inhibits glioma vasculogenic mimicry by controlling EphA2 expression. Mol Med Rep. 2018 Aug;18(2):1395-1404. doi: 10.3892/mmr.2018.9108. Epub 2018 May 31. PubMed PMID: 29901110; PubMed Central PMCID: PMC6072184.

5: Liu R, Tang JC, Pan MX, Zhuang Y, Zhang Y, Liao HB, Zhao D, Lei Y, Lei RX, Wang S, Liu AC, Qin XP, Chen J, Zhang ZF, Wan Q. ERK 1/2 Activation Mediates the Neuroprotective Effect of BpV(pic) in Focal Cerebral Ischemia-Reperfusion Injury. Neurochem Res. 2018 Jun 7. doi: 10.1007/s11064-018-2558-z. [Epub ahead of print] PubMed PMID: 29882124; PubMed Central PMCID: PMC6006215.

6: Liu R, Qin XP, Zhuang Y, Zhang Y, Liao HB, Tang JC, Pan MX, Zeng FF, Lei Y, Lei RX, Wang S, Liu AC, Chen J, Zhang ZF, Zhao D, Wu SL, Liu RZ, Wang ZF, Wan Q. Glioblastoma recurrence correlates with NLGN3 levels. Cancer Med. 2018 May 18. doi: 10.1002/cam4.1538. [Epub ahead of print] PubMed PMID: 29777576; PubMed Central PMCID: PMC6051187.

7: Zhao D, Chen J, Zhang Y, Liao HB, Zhang ZF, Zhuang Y, Pan MX, Tang JC, Liu R, Lei Y, Wang S, Qin XP, Feng YG, Chen Y, Wan Q. Glycine confers neuroprotection through PTEN/AKT signal pathway in experimental intracerebral hemorrhage. Biochem Biophys Res Commun. 2018 Jun 18;501(1):85-91. doi: 10.1016/j.bbrc.2018.04.171. Epub 2018 May 4. PubMed PMID: 29698679.

8: Fu R, Ding Y, Luo J, Huang KM, Tang XJ, Li DS, Guo SW. Ten-eleven translocation 1 regulates methylation of autophagy-related genes in human glioma. Neuroreport. 2018 Jun 13;29(9):731-738. doi: 10.1097/WNR.0000000000001024. PubMed PMID: 29659445.

9: Yan Y, Deng AP, Chen W, Ming YH, Zeng XT, Leng WD. Cumulative meta-analysis and trial sequential analysis of correlation between hOGG1 Ser326Cys polymorphism and the risk of head and neck squamous cell carcinoma. Oncotarget. 2018 Jan 6;9(16):13077-13087. doi: 10.18632/oncotarget.24055. eCollection 2018 Feb 27. PubMed PMID: 29560133; PubMed Central PMCID: PMC5849197.

10: Xue B, Ruan S, Xie P, Yan K, Gu Z, Meng N, Zhang J, Liu H, Lu J, Zuo S, Zhang H. Prospective analysis of glycemic variability in patients with severe traumatic brain injury: modified Leuven's adjustment process versus conventional adjustment process. J Int Med Res. 2018 Apr;46(4):1505-1516. doi: 10.1177/0300060517738396. Epub 2018 Jan 23. PubMed PMID: 29357724; PubMed Central PMCID: PMC6091849.

11: Zhang T, Yang X, Liu P, Zhou J, Luo J, Wang H, Li A, Zhou Y. Association between nonsteroidal anti-inflammatory drugs use and risk of central nervous system tumors: a dose-response meta analysis. Oncotarget. 2017 Oct 11;8(60):102486-102498. doi: 10.18632/oncotarget.21829. eCollection 2017 Nov 24. PubMed PMID: 29254264; PubMed Central PMCID: PMC5731974.

12: Zhang T, Yang X, Zhou J, Liu P, Wang H, Li A, Zhou Y. Benzodiazepine drug use and cancer risk: a dose-response meta analysis of prospective cohort studies. Oncotarget. 2017 Oct 19;8(60):102381-102391. doi: 10.18632/oncotarget.22057. eCollection 2017 Nov 24. PubMed PMID: 29254253; PubMed Central PMCID: PMC5731963.

13: Yang F, Wang Y, Li G, Xue J, Chen ZL, Jin F, Luo L, Zhou X, Ma Q, Cai X, Li HR, Zhao L. Effects of corilagin on alleviating cholestasis via farnesoid X receptor-associated pathways in vitro and in vivo. Br J Pharmacol. 2018 Mar;175(5):810-829. doi: 10.1111/bph.14126. Epub 2018 Jan 25. PubMed PMID: 29235094; PubMed Central PMCID: PMC5811621.

14: Hu J, Luo J, Wang H, Wang C, Sun X, Li A, Zhou Y, Liu Y, Chen Q. Association of TNF-α-3959T/C Gene Polymorphisms in the Chinese Population with Intracranial Aneurysms. J Mol Neurosci. 2017 Dec;63(3-4):349-354. doi: 10.1007/s12031-017-0985-y. Epub 2017 Oct 13. PubMed PMID: 29027627.

15: Yang L, Li C, Liang F, Fan Y, Zhang S. MiRNA-155 promotes proliferation by targeting caudal-type homeobox 1 (CDX1) in glioma cells. Biomed Pharmacother. 2017 Nov;95:1759-1764. doi: 10.1016/j.biopha.2017.08.088. Epub 2017 Oct 6. PubMed PMID: 28962081.

16: Liu Y, Tang ZG, Yang JQ, Zhou Y, Meng LH, Wang H, Li CL. Low concentration of quercetin antagonizes the invasion and angiogenesis of human glioblastoma U251 cells. Onco Targets Ther. 2017 Aug 11;10:4023-4028. doi: 10.2147/OTT.S136821. eCollection 2017. PubMed PMID: 28860810; PubMed Central PMCID: PMC5565384.

17: Fu W, Wang C, Zou L, Liu Q, Gan Y, Yan S, Song F, Wang Z, Lu Z, Cao S. Association between exposure to noise and risk of hypertension: a meta-analysis of observational epidemiological studies. J Hypertens. 2017 Dec;35(12):2358-2366. doi: 10.1097/HJH.0000000000001504. PubMed PMID: 28806352.

18: Sinha B, Wu Q, Li W, Tu Y, Sirianni AC, Chen Y, Jiang J, Zhang X, Chen W, Zhou S, Reiter RJ, Manning SM, Patel NJ, Aziz-Sultan AM, Inder TE, Friedlander RM, Fu J, Wang X. Protection of melatonin in experimental models of newborn hypoxic-ischemic brain injury through MT1 receptor. J Pineal Res. 2018 Jan;64(1). doi: 10.1111/jpi.12443. Epub 2017 Oct 23. PubMed PMID: 28796402.

19: Yang B, Qin J, Nie Y, Li Y, Chen Q. Brain-derived neurotrophic factor propeptide inhibits proliferation and induces apoptosis in C6 glioma cells. Neuroreport. 2017 Aug 16;28(12):726-730. doi: 10.1097/WNR.0000000000000828. PubMed PMID: 28640008; PubMed Central PMCID: PMC5502118.

20: Zeng XJ, Wang W, Zhao Z, Li M. Causes and preventive measures of symptomatic spinal epidural haematoma after spinal surgery. Int Orthop. 2017 Jul;41(7):1395-1403. doi: 10.1007/s00264-017-3506-2. Epub 2017 May 24. PubMed PMID: 28536800.

21: Liu Y, Tang ZG, Lin Y, Qu XG, Lv W, Wang GB, Li CL. Effects of quercetin on proliferation and migration of human glioblastoma U251 cells. Biomed Pharmacother. 2017 Aug;92:33-38. doi: 10.1016/j.biopha.2017.05.044. Epub 2017 May 18. PubMed PMID: 28528183.

22: Weng D, Jin X, Qin S, Lan X, Chen C, Sun X, She X, Dong C, An R. Radioimmunotherapy for CD133(+) colonic cancer stem cells inhibits tumor development in nude mice. Oncotarget. 2017 Jul 4;8(27):44004-44014. doi: 10.18632/oncotarget.16868. PubMed PMID: 28430648; PubMed Central PMCID: PMC5546457.

23: Lu JT, Deng AP, Song J, Zhang L, Luo J. Reappraisal of XRCC1 Arg194Trp polymorphism and glioma risk: a cumulative meta-analysis. Oncotarget. 2017 Mar 28;8(13):21599-21608. doi: 10.18632/oncotarget.15376. PubMed PMID: 28423490; PubMed Central PMCID: PMC5400609.

24: Ai Z, Li L, Fu R, Lu JM, He JD, Li S. Integrated Cox's model for predicting survival time of glioblastoma multiforme. Tumour Biol. 2017 Apr;39(4):1010428317694574. doi: 10.1177/1010428317694574. PubMed PMID: 28381184.

25: Fu R, Ding Y, Luo J, Yu L, Li CL, Li DS, Guo SW. TET1 exerts its tumour suppressor function by regulating autophagy in glioma cells. Biosci Rep. 2017 Nov 6;37(6). pii: BSR20160523. doi: 10.1042/BSR20160523. Print 2017 Dec 22. PubMed PMID: 28341638; PubMed Central PMCID: PMC5672083.

26: Hu S, Chen H, Zhang Y, Wang C, Liu K, Wang H, Luo J. MicroRNA-520c inhibits glioma cell migration and invasion by the suppression of transforming growth factor-β receptor type 2. Oncol Rep. 2017 Mar;37(3):1691-1697. doi: 10.3892/or.2017.5421. Epub 2017 Feb 3. PubMed PMID: 28184932.

27: Wen WS, Hu SL, Ai Z, Mou L, Lu JM, Li S. Methylated of genes behaving as potential biomarkers in evaluating malignant degree of glioblastoma. J Cell Physiol. 2017 Dec;232(12):3622-3630. doi: 10.1002/jcp.25831. Epub 2017 Mar 27. PubMed PMID: 28145562.

28: Gui H, Guo XR, Fang J, Ma SN, Wu G, Shen JH, Tu HJ, Li DS, Dai LJ, Ruan XZ. The Tumor-promoting Effects of FAM92A1-289 in Cervical Carcinoma Cells. Anticancer Res. 2016 Oct;36(10):5197-5204. PubMed PMID: 27798880.

29: Tang XJ, Huang KM, Gui H, Wang JJ, Lu JT, Dai LJ, Zhang L, Wang G. Pluronic-based micelle encapsulation potentiates myricetin-induced cytotoxicity in human glioblastoma cells. Int J Nanomedicine. 2016 Oct 3;11:4991-5002. eCollection 2016. PubMed PMID: 27757032; PubMed Central PMCID: PMC5055108.

30: Wei R, Cao G, Deng Z, Su J, Cai L. miR-140-5p attenuates chemotherapeutic drug-induced cell death by regulating autophagy through inositol 1,4,5-trisphosphate kinase 2 (IP3k2) in human osteosarcoma cells. Biosci Rep. 2016 Oct 14;36(5). pii: e00392. Print 2016 Oct. PubMed PMID: 27582507; PubMed Central PMCID: PMC5064456.

31: Guo XR, Yang ZS, Tang XJ, Zou DD, Gui H, Wang XL, Ma SN, Yuan YH, Fang J, Wang B, Zhang L, Sun XY, Warnock GL, Dai LJ, Tu HJ. The application of mRNA-based gene transfer in mesenchymal stem cell-mediated cytotoxicity of glioma cells. Oncotarget. 2016 Aug 23;7(34):55529-55542. doi: 10.18632/oncotarget.10835. PubMed PMID: 27487125; PubMed Central PMCID: PMC5342434.

32: Li HR, Li G, Li M, Zhang SL, Wang H, Luo T, Wu F, Dong JH, Guo YJ, Zhao L. Corilagin ameliorates schistosomiasis hepatic fibrosis through regulating IL-13 associated signal pathway in vitro and in vivo. Parasitology. 2016 Oct;143(12):1629-38. doi: 10.1017/S0031182016001128. Epub 2016 Jul 21. PubMed PMID: 27439782.

33: Tang XJ, Yang MH, Cao G, Lu JT, Luo J, Dai LJ, Huang KM, Zhang LI. Protective effect of microRNA-138 against cerebral ischemia/reperfusion injury in rats. Exp Ther Med. 2016 Mar;11(3):1045-1050. Epub 2016 Jan 22. PubMed PMID: 26998035; PubMed Central PMCID: PMC4774489.

34: Chen X, Luo J, Meng L, Pan T, Zhao B, Tang ZG, Dai Y. Dracorhodin perchlorate induces the apoptosis of glioma cells. Oncol Rep. 2016 Apr;35(4):2364-72. doi: 10.3892/or.2016.4612. Epub 2016 Feb 4. PubMed PMID: 26846469.

35: Cai Z, Wang C, He W, Tu H, Tang Z, Xiao M, Yan LJ. Cerebral small vessel disease and Alzheimer's disease. Clin Interv Aging. 2015 Oct 23;10:1695-704. doi: 10.2147/CIA.S90871. eCollection 2015. Review. PubMed PMID: 26604717; PubMed Central PMCID: PMC4629951.

36: Tang XJ, Sun XY, Huang KM, Zhang L, Yang ZS, Zou DD, Wang B, Warnock GL, Dai LJ, Luo J. Therapeutic potential of CAR-T cell-derived exosomes: a cell-free modality for targeted cancer therapy. Oncotarget. 2015 Dec 29;6(42):44179-90. doi: 10.18632/oncotarget.6175. Review. PubMed PMID: 26496034; PubMed Central PMCID: PMC4792550.

37: Wu Q, Chen W, Sinha B, Tu Y, Manning S, Thomas N, Zhou S, Jiang H, Ma H, Kroessler DA, Yao J, Li Z, Inder TE, Wang X. Neuroprotective agents for neonatal hypoxic-ischemic brain injury. Drug Discov Today. 2015 Nov;20(11):1372-81. doi: 10.1016/j.drudis.2015.09.001. Epub 2015 Sep 7. Review. PubMed PMID: 26360053.

38: Niu YM, Du XY, Lu MY, Xu QL, Luo J, Shen M. Significant association between functional microRNA polymorphisms and head and neck cancer susceptibility: a comprehensive meta-analysis. Sci Rep. 2015 Aug 17;5:12972. doi: 10.1038/srep12972. PubMed PMID: 26277865; PubMed Central PMCID: PMC4538372.

39: Wang G, Wang J, Zhao H, Wang J, Tony To SS. The role of Myc and let-7a in glioblastoma, glucose metabolism and response to therapy. Arch Biochem Biophys. 2015 Aug 15;580:84-92. doi: 10.1016/ Epub 2015 Jul 4. Review. PubMed PMID: 26151775.

40: Xu CS, Wang ZF, Huang XD, Dai LM, Cao CJ, Li ZQ. Involvement of ROS-alpha v beta 3 integrin-FAK/Pyk2 in the inhibitory effect of melatonin on U251 glioma cell migration and invasion under hypoxia. J Transl Med. 2015 Mar 20;13:95. doi: 10.1186/s12967-015-0454-8. PubMed PMID: 25889845; PubMed Central PMCID: PMC4371719.

41: Niu YM, Deng MH, Chen W, Zeng XT, Luo J. MTHFR C677T gene polymorphism and head and neck cancer risk: a meta-analysis based on 23 publications. Dis Markers. 2015;2015:681313. doi: 10.1155/2015/681313. Epub 2015 Jan 31. Review. PubMed PMID: 25802478; PubMed Central PMCID: PMC4329770.

42: Zeng XT, Lu JT, Tang XJ, Weng H, Luo J. Association of methionine synthase rs1801394 and methionine synthase reductase rs1805087 polymorphisms with meningioma in adults: A meta-analysis. Biomed Rep. 2014 May;2(3):432-436. Epub 2014 Mar 12. PubMed PMID: 24748989; PubMed Central PMCID: PMC3990204.

43: Yang ZS, Tang XJ, Guo XR, Zou DD, Sun XY, Feng JB, Luo J, Dai LJ, Warnock GL. Cancer cell-oriented migration of mesenchymal stem cells engineered with an anticancer gene (PTEN): an imaging demonstration. Onco Targets Ther. 2014 Mar 17;7:441-6. doi: 10.2147/OTT.S59227. eCollection 2014. PubMed PMID: 24669193; PubMed Central PMCID: PMC3962313.

44: Jiang T, Tang GF, Lin Y, Peng XX, Zhang X, Zhai XW, Peng X, Yang JQ, Huang HE, Wu NF, Chen XJ, Xing HX, Su TY, Wang ZC. Prevalence estimates for primary brain tumors in China: a multi-center cross-sectional study. Chin Med J (Engl). 2011 Sep;124(17):2578-83. PubMed PMID: 22040406.

45: Huang KM, Peng M, Feng YQ, Huang H, Tu HJ, Luo J, Zhang L, Yuan XH, Wang LC. Cryosurgery and rhTNF-α play synergistic effects on a rat cortex C6 glioma model. Cryobiology. 2012 Feb;64(1):43-9. doi: 10.1016/j.cryobiol.2011.09.137. Epub 2011 Sep 29. PubMed PMID: 21982953.

46: Zeng Y, Xiong M, Yu H, He N, Wang Z, Liu Z, Han H, Chen S. [Comparative study on microplate and anchor fixation in open-door cervical expansive laminoplasty]. Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2011 Aug;25(8):946-50. Chinese. PubMed PMID: 21923022.

47: Chen LJ, Zhang FG, Li J, Song HX, Zhou LB, Yao BC, Li F, Li WC. Expression of calcitonin gene-related peptide in anterior and posterior horns of the spinal cord after brachial plexus injury. J Clin Neurosci. 2010 Jan;17(1):87-91. doi: 10.1016/j.jocn.2009.03.042. Epub 2009 Dec 6. PubMed PMID: 19969463.

48: Luo J, Feng J, Lu J, Wang Y, Tang X, Xie F, Li W. Aberrant methylation profile of 14-3-3 sigma and its reduced transcription/expression levels in Chinese sporadic female breast carcinogenesis. Med Oncol. 2010 Sep;27(3):791-7. doi: 10.1007/s12032-009-9287-8. Epub 2009 Aug 14. PubMed PMID: 19685192.

49: Wang QX, Wang XY, Fu NA, Liu JY, Yao SL. Stellate ganglion block inhibits formalin-induced nociceptive responses: mechanism of action. Eur J Anaesthesiol. 2005 Dec;22(12):913-8. PubMed PMID: 16318661.

50: Wang H, Tu HJ, Qin J, Li XJ, Huang KM, Zhou ZM, Wang LC. [Effect of cryotherapy and 5-fluorouracil on apoptosis of G422 glioma cells]. Ai Zheng. 2004 Apr;23(4):412-5. Chinese. PubMed PMID: 15087029.

Meng ZD, Wang XL, Du TP, Wang Y, Qin GF, Zhao HB, Chen YJ, Tian B. Effects of RNA interference-mediated E-selectin gene silencing on cell adhesion molecule expression and cell-cell adhesion in vascular endothelial cells in mice with immunologic contact urticaria. J Cell Biochem. 2018 Sep 11. doi: 10.1002/jcb.27150. [Epub ahead of print] PubMed PMID: 30206961.
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